InvivoGentlrl-fmdpMDP FITC重慶市華雅干細(xì)胞

InvivoGen tlrl-fmdp MDP FITC
MDP FITC

FITC-labeled MDP

Muramyl dipeptide (MDP) is the minimal bioactive peptidoglycan motif common to all bacteria, the essential structure required for adjuvant activity in vaccines. MDP has been shown to be recognized by NOD2, but not TLR2, nor TLR2/1 or TLR2/6 associations [1,2]. This recognition is highly stereospecific of the L-D isomer, excluding any reaction to the D-D or L-L analogs [2]. The potent adjuvant activity of MDP may also be linked to an activation of the CIAS1/NALP3/Cryopyrin inflammasome [3].

MDP FITC is coupled via a 6-aminohexanoic acid spacer molecule at the C6 position of the muric acid. This spacer linker arm minimizes potential steric hindrance effects.

Specificity: NOD2 agonist
Working concentration: 100 ng -10 μg/ml
Endotoxin level: EndoFit™ (<0.001 EU/μg)
Solubility: DMSO (10 mg/ml)
Molecular weight: 995.02

Spectral Properties of FITC
Excitation λ max: 495 nm
Emission λ max: 525 nm

Contents

• 500 μg Muramyl dipeptide (MDP) labeled with FITC
 • 2 ml sterile endotoxin-free water

MDP FITC is provided as a lyophilized powder and shipped at room temperature. Store at -20°C. Upon resuspension, aliquote MDP FITC and store at -20°C. Product is stable 6 months at -20°C when properly stored. Avoid repeated freeze-thaw cycles.

Ref.

1. Girardin SE. et al., 2003. Nod2 is a general sensor of peptidoglycan through muramyl dipeptide (MDP) detection. J Biol Chem. 278(11):8869-72.
2. Inohara N. et al., 2003. Host recognition of bacterial muramyl dipeptide mediated through NOD2. Implications for Crohn's disease.J Biol Chem. 278(8):5509-12.
3. Martinon F. et al., 2004. Identification of bacterial muramyl dipeptide as activator of the NALP3/cryopyrin inflammasome. Curr Biol. 14(21):1929-34.

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