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Molecular Biology Reagents
RNA ISOLATION:
1. RNAzol B®
Complete and ready-to-use reagent for isolation of total RNA from samples of human, animal, plant, bacterial and viral origin.
Cat # Quantity
WAK-CS105 200 ml
2. TRI Reagent®
A reagent for the single-step isolation of total RNA or simultaneous isolation of RNA, DNA and proteins from tissues, cells cultured in monolayer or cell pellets.1 ml of TRI Reagent processes up to 100 mg tissue or 107 cells.
Cat # Quantity
TR 118 200 ml
3. TRI Reagent® BD
A reagent specifically designed for the single-step isolation of total RNAor simultaneous isolation of RNA, DNA and proteins from whole blood, plasma or serum. 0.75 ml of TRI Reagent® BD processes 0.25 ml of blood derivatives.
Cat # Quantity
TB 126 200 ml
4. TRI Reagent® LS
A reagent for the single-step isolation of total RNA or simultaneous isolation of RNA, DNA and proteins from liquid samples such as cell suspensions, serum, CSF and amniotic fluid. 0.75 ml of TRI Reagent® LS processes 0.25 ml of liquid samples.
Cat # Quantity
TS 120 200 ml
5. BCP
BCP is a molecular biology grade 1-bromo-3-chloropropane.This new phase separation reagent substitutes for highly volatileand toxic chloroform in the TRI Reagent procedures.
Cat # Quantity
BP 151 200 ml
6. FORMAzol®
Stabilized formamide for use as an RNA solubilizer instead of DEPC-treated water.It allows storage of RNA at -20C, protects from degradation by Rnase,and simplifies sample preparation for northern analysis.
Cat # Quantity
FO 121 25 ml
7. Oligo (dT)-Cellulose Columns
A set of 5 reusable columns and one vial of PolyAcryl Carrier for isolation of poly A+ RNA.
Cat # Quantity
OT 125 set
8. PolyAcryl Carrier
Molecular biology grade acryl polymer for use in precipitation of RNA and DNA.
Cat # Quantity
PC 152 5 ml
9. High Salt Precipitation Solution
For use in the TRI Reagent procedure, to eliminate polysaccharideand proteoglycan contamination from RNA samples.
Cat # Quantity
PS 161 100 ml
10. Phosphate Buffer for Spectrophotometry
100X stock solution for use in the spectrophotometric analysis of RNA and DNA.
Cat # Quantity
SP 130 100 ml
DNA ISOLATION:
1. DNAzol®
Genomic DNA isolation reagent for use with tissues, cells and liquids. Non-toxic, no phenol use, the isolation is fast (10-30 min), simple and reliable. 1 ml of DNAzol® isolates DNA from 25-50 mg tissue or 1-3 x 107 cells.
Cat # Quantity
DN 127 200 ml
2. DNAzol® ES
Extra Strength DNAzol® reagent for isolation of genomic DNA from plants. Non-toxic. 1.5 ml DNAzol® ES isolates DNA from 0.5 g of plant sample.
Cat # Quantity
DN 128 200 ml
3. DNAzol®BD
A DNAzol® reagent specifically designed for isolation of genomic DNA from whole blood. Non-toxic. 1 ml of DNAzol® BD isolates DNA from 0.5 ml of whole blood.
Cat # Quantity
DN 129 200 ml
4. Bactozol™ Kit
A kit for the isolation of bacterial DNA. Kit includes 10X Bactozol™ Enzyme Solution, Bactozol Enzyme Solution Dilution Buffer and DNAzol® sufficient for 125 isolations, each yielding up to 40 µg bacterial DNA.
Cat # Quantity
BA 154 125 reactions
5. Bactozol™ Enzyme Solution
A solution stable at room temperature containing activated lysozyme for the effective lysis of a broad range of bacterial specimens. Sufficient for 125 lysis reactions, each with up to 40 µg bacterial DNA.
Cat # Quantity
BZ 160 125 reactions
ELECTROPHORESIS & HYBRIDIZATION:
1. Methylene Blue Stain
Staining solution for qualitative and quantitative analysis of RNA and DNAimmobilized on hybridization membranes, for use in Southern and northernblotting, detects 25 ng of RNA or DNA.
Cat # Quantity
MB 119 500 ml
2. Background Quencher™
10X concentrated blocking reagent that prevents non-specificbinding of hybridization probes.
Cat # Quantity
BQ 112 30 ml
60 ml
3. Molecular Biology Agarose
Suitable for electrophoretic separation of nucleic acids in molecular biology applications. RNase-free, DNase-free ultra pure agarose.
Cat # Quantity
MA 124 100 g
200 g
4. Super Agarose
Aqueous agarose suspension designed for optimal electrophoretic separationof nucleic acids. This 10% stock suspension can be diluted with a variety of electrophoresis buffers. RNase-free and DNase-free.
Cat # Quantity
SA 153 200 ml
500 ml
5. High Efficiency Hybridization System
A complete hybridization solution containing dextran sulfate and Background Quencher™.
A Component of Super Hyb Kit.
Cat # Quantity
HS 114 100 ml
250 ml
500 ml
6. High Efficiency Hybridization System - with 50% formamide
A Component of Super Hyb Kit.
Cat # Quantity
HS 114 F 100 ml
250 ml
500 ml
7. Washing & Pre-Hyb Solution
10X concentrated washing and pre-hyb solution for use in northern and Southern blotting.
A Component of Super Hyb Kit.
Cat # Quantity
WP 117 500 ml
1000 ml
8. Super HYBTMKIT
A kit providing the highest signal intensity and low background in Northern, Southern and dotblot hybridizations. Contains solutions for hybridizations, pre- and post- hybridization washes.
Cat # Quantity
SK 116-SM
SK 116-LG 10-20 hybs
25-50 hybs
9. SUPER HYBTMKIT with 50% formamide
Cat # Quantity
SK 116F-SM
SK 116F-LG 10-20 hybs
25-50 hybs
DISPOSABLE POLYPROPYLENE CENTRIFUGE TUBES
1. Series A Tubes have standard snap-in caps
Cat # Quantity
PP 141A
PP 142A
PP 143A
PP 144A 5 ml (13 x 75 mm) 125/box
8 ml (13 x 100 mm) 125/box
13 ml (17 x 95 mm) 75/box
20 ml (21 x 100 mm) 50/box
2. Series B Tubes have Double-edge caps
Cat # Quantity
PP 141B
PP 142B
PP 143B 5 ml (13 x 75 mm) 125/box
8 ml (13 x 100 mm) 125/box
13 ml (17 x 95 mm) 75/box
3. Disposable Polypropylene Microfuge tubes
Disposable Polypropylene Microfuge Tubes are tested in RNA, DNA and protein isolation using TRI Reagents, and withstand centrifugal forces of 12,000 g. The tubes fit eppendorf type centrifuges and have screw caps to reduce the hazard of phenol contamination. Made of ultra-clear polypropylene.
Cat # Quantity
PP 131
PP 132 2 ml non-sterile 200/box
2 ml sterile 200/box
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